Uji Aktivitas Anti Jamur Ekstrak Etanol Biji Alpukat (Persea americana Mill) Terhadap Jamur Kulit Tricophyton rubrum ATCC 28188, Epidermophyton floccosum ATCC 50266 dan Micospprum canis ATCC 32699)
DOI:
https://doi.org/10.32524/jksp.v4i2.264Kata Kunci:
Avocado, Phytochemicals, DermatophytosisAbstrak
Antifungal activity test of avocado seed extract (Persea americana Mill) against fungi that cause dermatophytosis skin disease, namely Trichophyton rubrum ATCC 28188, Epidermophyton floccosum ATCC 50266 and Microsporum canis ATCC 32699. Phytochemical test results of avocado seeds contain phenolic compounds, saponins, alkaloids, flavonoids. The antifungal activity test of ethanol extract of avocado seeds used the agar diffusion method, with a concentration of 26,25%, 48,75%, 75%, and the positive control used was ketoconazole and ethanol distillate negative control. The results showed that the diameter of the zone of inhibition against the fungus Trichophyton rubrum ATCC 28188 with an inhibition zone diameter of 7.5 mm, 9.8 mm, 12 mm, against the fungus Epidermophyton floccosum ATCC 50266, the inhibition zone diameter was 7.4 mm, 9, 7 mm, 11.7 mm and against fungi Microsporum canis ATCC 32699 the inhibition zone was 8.4 mm, 10.1 mm, 13.4 mm. It can be concluded that the ethanol extract of avocado seeds has antifungal activity against. Trichophyton rubrum ATCC 28188, Epidermophyton floccosum ATCC 50266 and Microsporum canis ATCC 32699 The greatest antifungal activity was against Microsporum canis at a concentration of 75% with a diameter of 13.4 mm in the strong category
Referensi
Andi Nur Fitriani Abu bakar., Aisyah., dan Maswati Baharuddin (2014). Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana) dan Uji Toksisitas Terhadap Artermia Salina Leach. Makasar : UIN Alauddin
Akhsin Zulkoloni (2010). Parasitologi. Yogyakarta : Muha medika. p. 61-70
Berman,J.(2012).Taxonomicguide to infection diseases. USA: Academic Press
Brooks, G.F., Butel, J.S., dan Ornston, L.C. (Persea americana Mill) terhadap jamur uji jamur Trichophyton rubrum, Epidermophyton floccosum dan (2013). Mikrobiologi Kedokteran. Jakarta: EGC.
Departemen Kesehatan RI. (1995). Farmakope Indonesia
Departemen Kesehatan RI. (2000). Parameter standar umum ekstrak tumbuhan obat. Edisi I. Jakarta: Direktorat Jendral POM- RI
Goodman, dan Gillman. (2008). Manual of Pharmacology and Terapeutics. United States of America: the MeGraw-Hill Companics.
Harmita, dan Radji, M. (2008). Buku ajaran analisi hayati. Jakrta: EGC.
Hariana, A. (2009). Tumbuhan Obat dan Khasiatnya Seri 3. Cet.5. Penebar Swadaya. Jakarta.
Herbie, T. (2015). Kitab tanaman berkhasiat obat 226 tumbuhan obat untuk americana Mill. Jurnal Mipa UNSRAT. Online 1 24-28
Pratiwi, ST. (2008). Mikrobiologi Farmasi. Jakarta: Erlangga.
P. (2010). Contents and antibacterial activity of flavonoids extracted from leaves of Psidium guajava. Journal of Medicinal Plants Research. 4(5):393-396. http://www.itis.gov. Diakses pada 27 Setiadi, (2007). Konsep dan Penulisan Riset januari 2021
Harti, A.S. (2015). Mikrobiologi kesehatan.Yogyakarta: CV. Andi Offset Khoiriyah,
A., Farisa, A., Ahmad, S., dan Eddy, B. (2012). Aplikasi Pendukung Keputusan Epidemiologi Resisten Bakteri Menggunakan Metode Dilusi di RSUD dr. Soetomo. Surabaya: Institut Teknologi Sepuluh November.
Simes, J. J. H., Tracey, J.G., Webb, L. J., & Dunstand, W. J. (1959). An Australian Phytochemical Survey III: Saponins in Eastern Australian Flowering Plants. CSIRO, 281, 5–8.
Suriawiria. (1995). Pengantar mikrobiologi umum. Bandung: Angkasa.
